Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2892] to PON1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PON1 antibody [EPR2892] - BSA and Azide free
    See all PON1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2892] to PON1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247559 is the carrier-free version of ab92466 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247559 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Western blot - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    All lanes : Anti-PON1 antibody [EPR2892] (ab92466) at 1/1000 dilution

    Lane 1 : Human plasma cell lysate
    Lane 2 : ovarian cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
    Lane 2 : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 40 kDa



    This data was developed using ab92466, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)

    This data was developed using ab92466, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)

    This data was developed using ab92466, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)

    This data was developed using ab92466, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue labelling PON1 with ab92466 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 min. The section was incubated with ab92466 for 30 mins at room temperature. Cytoplasmic localization of PON1 visualized using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    This data was developed using ab92466, the same antibody clone in a different buffer formulation.ab92466, at 1/100 dilution, staining PON1 in formalin-fixed, paraffin-embedded Human liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    This data was developed using ab92466, the same antibody clone in a different buffer formulation.ab92466 showing negative staining in Skeletal muscle tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    This data was developed using ab92466, the same antibody clone in a different buffer formulation.ab92466 showing negative staining in Normal colon tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    This data was developed using ab92466, the same antibody clone in a different buffer formulation.ab92466 showing negative staining in Normal breast tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    This data was developed using ab92466, the same antibody clone in a different buffer formulation.ab92466 showing negative staining in Normal brain tissue.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)
    Anti-PON1 antibody [EPR2892] - BSA and Azide free (ab247559)

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