Anti-JAK2 antibody (ab245303)
Key features and details
- Rabbit polyclonal to JAK2
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-JAK2 antibody
See all JAK2 primary antibodies -
Description
Rabbit polyclonal to JAK2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human JAK2 aa 1082-1132. The exact sequence is proprietary. (NP_004963.1).
Database link: O60674 -
Positive control
- WB: Jurkat and HeLa whole cell lysates. IP: Jurkat whole cell lysate. IHC-P: Human prostate carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245303 was affinity purified using an epitope specific to JAK2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-JAK2 antibody (ab245303)All lanes : Anti-JAK2 antibody (ab245303) at 0.1 µg/ml
Lane 1 : Jurkat (humanT cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 131 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-JAK2 antibody (ab245303)JAK2 was immunoprecipitated from Jurkat (humanT cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245303 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245303 at 1 µg/ml.
Lane 1: ab245303 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (ab245303)Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for JAK2 using ab245303 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
