Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR108(2)] to JAK2 - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free
    See all JAK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR108(2)] to JAK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549 and K562 cell lysate. IP: K562 cell lysate. ICC/IF: K562, Ramos and Jurkat cells.

  • General notes

    Ab170718 is the carrier-free version of ab108596. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab170718 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)
    Western blot - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)
    All lanes : Anti-JAK2 antibody [EPR108(2)] (ab108596) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : JAK2 knockout A549 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 131 kDa
    Observed band size: 131 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab108596).

      Lanes 1- 4: Merged signal (red and green). Green - ab108596 observed at 131 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab108596 was shown to react with JAK2 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab267113 (knockout cell lysate ab256963) was used. Wild-type A549 and JAK2 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108596 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)
    Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)

    ab108596 at 1/500 dilution (3.0µg/ml) immunoprecipitating JAK2 in K562 (Human chronic myelogenous leukemia lymphoblast)  cell lysate.

    Lane 1 (input): K562(Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg

    Lane 2 (+): K562 whole cell lysate, 350µg  + ab108596, 2µg

    Lane 3 (-): K562 cell lysate, 350µg  + rabbit IgG (ab172730), 2µg

    For western blotting, ab131366 VeriBlot for IP (HRP) was used at 1/1000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108596).

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)
    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)

    Immunocytochemistry/ Immunofluorescence analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling JAK2 with purified ab108596 at 1/150 dilution (8.5μg/ml). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, a goat anti-rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the counter stain at 1/200 (2.5 μg/ml). PBS instead of the primary antibody was the negative control. DAPI was used as a nuclear counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108596).

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)
    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)

    Immunocytochemistry/Immunofluorescence analysis of Ramos (Human Burkitt's lymphoma) cells labelling JAK2 with unpurified 108596 at 1/300 (7.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    Confocal image showing nuclear and cytoplasmic staining on Ramos cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108596).

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)
    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cells from peripheral blood) cells labelling JAK2 with unpurified ab108596 at 1/300 (7.0 μg/mL). Cells were fixed with 4% Paraformaldehydeand permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    Confocal image showing nuclear and cytoplasmic staining on Jurkat cell line. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108596).

  • Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)
    Anti-JAK2 antibody [EPR108(2)] - BSA and Azide free (ab170718)

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