Anti-BIN1 antibody (ab264303)
Key features and details
- Rabbit polyclonal to BIN1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BIN1 antibody
See all BIN1 primary antibodies -
Description
Rabbit polyclonal to BIN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BIN1 aa 543-593. The exact sequence is proprietary.
Database link: O00499 -
Positive control
- IHC-P: Human ovarian carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody (ab264303)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling BIN1 with ab264303 at 1/1,000 dilution. Detection: DAB.
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Western blot - Anti-BIN1 antibody (ab264303)All lanes : Anti-BIN1 antibody (ab264303) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 65 kDa
Exposure time: 3 minutes4-8% SDS-PAGE
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Immunoprecipitation - Anti-BIN1 antibody (ab264303)BIN1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264303 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264303 at 1 µg/ml.
Lane 1: ab264303 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 mins.
