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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] - BSA and Azide free (ab247355)

Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] - BSA and Azide free (ab247355)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2136Y] to PDGFR beta (phospho Y857) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] - BSA and Azide free
    See all PDGFR beta primary antibodies
  • Description

    Rabbit monoclonal [EP2136Y] to PDGFR beta (phospho Y857) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody detects PDGFR beta phosphorylated at Tyrosine 857.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247355 is the carrier-free version of ab62367 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247355 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP2136Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF
    • Cancer
    • Growth factors
    • PDGF
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Images

  • Western blot - Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] - BSA and Azide free (ab247355)
    Western blot - Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] - BSA and Azide free (ab247355)
    All lanes : Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] (ab62367) at 1/1000 dilution

    Lane 1 : NIH3T3 cell lysates (untreated)
    Lane 2 : NIH3T3 cell lysates treated with PDGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit, HRP labeled, at 1/2000 dilution

    Predicted band size: 123 kDa
    Observed band size: 190 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab62367, the same antibody clone in a different buffer formulation.

     

  • Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] - BSA and Azide free (ab247355)
    Anti-PDGFR beta (phospho Y857) antibody [EP2136Y] - BSA and Azide free (ab247355)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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