Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 16, 2021

Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21744] to PDGFR beta (phospho Y751)
Suitable for: Dot blot, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-PDGFR beta (phospho Y751) antibody [EPR21744]
See all PDGFR beta primary antibodies
Description

Rabbit monoclonal [EPR21744] to PDGFR beta (phospho Y751)
Host species

Rabbit
Tested Applications & Species

Application Species
Dot
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: IMR-90 (treated with 50 ng/ml PDGF-BB for 5 minutes), NIH/3T3 (treated with 50 ng/ml PDGF-BB for 5 minutes) and C6 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Dot	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21744
Isotype

IgG
Research areas

Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)

All lanes : Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534) at 1/1000 dilution

Lane 1 : Untreated IMR-90 (human lung fibroblast cell line) whole cell lysate
Lane 2 : IMR-90 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
Lane 3 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : NIH/3T3 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate
Lane 5 : Untreated C6 (rat glial tumor glial cell line) whole cell lysate
Lane 6 : C6 (treated with 50 ng/ml PDGF-BB for 5 minutes) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 124 kDa
Observed band size: 124,190 kDa
why is the actual band size different from the predicted?

Exposure times : Lanes 1-4: 26 seconds, Lanes 5 & 6: 3 minutes.

Blocking and dilution buffer: 1% BSA/TBST.

Expression of PDGFR beta (phospho Y751) is induced by PDGF-BB. The antibody recognises both the glycosylated and unglycosylated protein (PMID: 9307305).
Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)

Dot blot analysis of PDGFR beta (phospho Y751) labeled with ab218534 at 1/1000 dilution.

Lane 1: Human PDGFR beta (phospho Y751) peptide.
Lane 2: Human PDGFR beta non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)

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