Rat L-Selectin ELISA Kit (ab263876)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.77 ng/ml
- Range: 1.6 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat L-Selectin ELISA Kit
See all CD62L kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 6.5% Inter-assay Sample n Mean SD CV% Plasma 3 10.9% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.77 ng/ml -
Range
1.6 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 120 119% - 120% Serum 90 86% - 95% EDTA Plasma 88 82% - 95% Cit plasma 89 84% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat L-Selectin ELISA Kit (ab263876) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of L-Selectin protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat L-Selectin with 0.77 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
L-Selectin, also referred to as CD62L and Leukocyte adhesion molecule 1, is a calcium-dependent lectin encoded by the gene SELL. L-Selectin binds to glycoproteins to mediate cell adhesion on neighboring cells. In peripheral lymph nodes, L-selectin mediates the adherence of lymphocytes to endothelial cells with high endothelial venules. L-Selectin is composed of several domains, one homologous to lectins, an epidermal growth factor domain, and two to repeat units found In C3/C4 binding proteins.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat L-Selectin Capture Antibody 1 x 600µl 10X Rat L-Selectin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat L-Selectin Lyophilized Recombinant Protein 2 vials Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. -
Tissue specificity
Expressed in B cell lines and T lymphocytes. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- A.11
- AI528707
- CD62 antigen ligand
see all -
Database links
- Entrez Gene: 29259 Rat
- SwissProt: P30836 Rat
- Unigene: 10461 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat L-Selectin standard curve in Sample Diluent NS.The L-Selectin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native L-Selectin in serum, rat plasma (EDTA), and plasma (citrate) samples.The concentrations of L-Selectin were measured in duplicates, interpolated from the L-Selectin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 100%, and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L-Selectin concentration was determined to be 41.8 ng/mL in serum, 30 ng/mL in plasma (EDTA), and 25.1 ng/mL in plasma (citrate).
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Interpolated concentrations of native L-Selectin in rat spleen cell supernatant samples.The concentrations of L-Selectin were measured in duplicates, interpolated from the L-Selectin standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L-Selectin concentration was determined to be 44.1 ng/mL in spleen cell supernatant.
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Sandwich ELISA - Rat L-Selectin ELISA Kit (ab263876)To learn more about the advantages of recombinant antibodies see here.
