Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PD-L1 antibody [EPR20529] - BSA and Azide free (ab223141)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20529] to PD-L1 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PD-L1 antibody [EPR20529] - BSA and Azide free
    See all PD-L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20529] to PD-L1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta lysate; whole cell lysate from RAW 264.7 cells treated with 100ng/ml Interferon-gamma for 48 hours. IP: Mouse placenta lysate. ICC: RAW 264.7 cells treated with IFN-? (100 ng/ml) for 24 hours.

  • General notes

    Ab223141 is the carrier-free version of ab213480. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223141 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] - BSA and Azide free (ab223141)
    Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] - BSA and Azide free (ab223141)

    Ab213480 staining PD-L1 in RAW264.7 from Mouse macrophage cell line transformed with Abelson murine leukemia virus by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% Methanol. Samples were incubated with ab213480 at 0.4 μg/ml. AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(ab196889) was used as counterstain at 2.5 μg/ml. DAPI used as nuclear counterstain. Confocal image showing membranous staining on RAW 264.7 cells treated with IFN-γ (100 ng/ml) for 24 hours.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213480).

  • Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] - BSA and Azide free (ab223141)
    Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] - BSA and Azide free (ab223141)

    PD-L1 was immunoprecipitated from 0.35 mg of Mouse placenta lysate with ab213480 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213480 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse placenta lysate, 10 µg (Input).

    Lane 2: ab213480 IP in mouse placenta lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213480 in mouse placenta lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213480).

  • Anti-PD-L1 antibody [EPR20529] - BSA and Azide free (ab223141)
    Anti-PD-L1 antibody [EPR20529] - BSA and Azide free (ab223141)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PD-L1 antibody [EPR20529] - BSA and Azide free (ab223141)

© 2021 Astana Biomed Group LLP. All rights reserved.