Anti-Paxillin antibody [M107] (ab23510)
![Anti-Paxillin antibody [M107] (ab23510)](https://www.abcam.com/ps/products/23/ab23510/Images/ab23510-353555-antipaxillinantibodym107westernblotknockouta431pxnu87mgpc3humanlysates23510gdb.jpg "Anti-Paxillin antibody [M107] (ab23510)")
Key features and details
- Mouse monoclonal [M107] to Paxillin
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Paxillin antibody [M107]
See all Paxillin primary antibodies -
Description
Mouse monoclonal [M107] to Paxillin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein (Human).
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Positive control
- A431 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M107 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Paxillin antibody [M107] (ab23510)All lanes : Anti-Paxillin antibody [M107] (ab23510) at 1/1000 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : PXN knockout A431 whole cell lysate
Lane 3 : U-87 MG whole cell lysate
Lane 4 : PC-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 65 kDaLanes 1 - 4: Merged signal (red and green). Green - ab23510 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab23510 was shown to recognize PXN in wild-type A431 cells as signal was lost at the expected MW in PXN knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PXN knockout samples were subjected to SDS-PAGE. Ab23510 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Paxillin antibody [M107] (ab23510)](https://www.abcam.com/ps/products/23/ab23510/Images/ab23510-33350-anti-paxillin-antibody-m107-western-blot.jpg)
Western blot - Anti-Paxillin antibody [M107] (ab23510)Lanes 1-2 : Anti-Paxillin antibody [M107] (ab23510)
Lanes 3-4 : Anti-Paxillin (Ser178) antibody
Lanes 1 & 3 : A431 cells (20 ug/lane) serum starved overnight
Lanes 2 & 4 : A431 cells (20 ug/lane) serum treated with EGF (100 ng/ml) for 5 min.
Predicted band size: 65 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
