Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5678] to p53 (phospho T377) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR5678] to p53 (phospho T377) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects p53 phosphorylated at Threonine 377. 

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248500 is the carrier-free version of ab133451 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248500 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)
    Western blot - Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)
    All lanes : Anti-p53 (phospho T377) antibody [EPR5678] (ab133451) at 1/20000 dilution

    Lane 1 : HEK293 (human embryonic kidney) treated with Okadaic acid and Calyculin A whole cell lysate
    Lane 2 : Untreated HEK293 (human embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab133451, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)
    Western blot - Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)
    All lanes : Anti-p53 (phospho T377) antibody [EPR5678] (ab133451) at 1/10000 dilution

    Lane 1 : Lysate of 293T cells, untreated
    Lane 2 : Lysate of 293T cells treated with okadaic acid and calyculin A.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat ant-rabbit antibody at 1/2000 dilution

    Predicted band size: 43 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    This data was developed using ab133451, the same antibody clone in a different buffer formulation.

  • Dot Blot - Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)
    Dot Blot - Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)
    This data was developed using ab133451, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: p53 (pT377) phospho peptide Lane 2 sample: p53 non-phospho peptide Exposure time: 3 minutes
  • Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)
    Anti-p53 (phospho T377) antibody [EPR5678] - BSA and Azide free (ab248500)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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