Anti-VRK1 antibody [5D1] - N-terminal (ab171933)
![Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-397679-anti-vrk1-antibody-5d1-n-terminal-western-blot-human-knockout-lysate.jpg "Anti-VRK1 antibody [5D1] - N-terminal (ab171933)")
Key features and details
- Mouse monoclonal [5D1] to VRK1 - N-terminal
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-VRK1 antibody [5D1] - N-terminal
See all VRK1 primary antibodies -
Description
Mouse monoclonal [5D1] to VRK1 - N-terminal -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human VRK1 aa 1-19 (N terminal) (Cysteine residue).
Sequence:MPRVKAAQAGRQSSAKRHL-C
Database link: Q99986 -
Positive control
- WB: HEK293T, HeLa, TIG-1 and U2OS cell lysates. ICC/IF: interphase and metaphase HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
5D1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : VRK1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab171933 observed at 50 kDa. Red - loading control ab181602 observed at 36 kDa.
ab171933 Anti-VRK1 antibody [5D1] - N-terminal was shown to specifically react with VRK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266257 (knockout cell lysate ab258283) was used. Wild-type and VRK1 knockout samples were subjected to SDS-PAGE. ab171933 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-196992-anti-vrk1-antibody-5d1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Immunofluorescent analysis of paraformaldehyde-fixed interphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).
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![Western blot - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-196989-anti-vrk1-antibody-5d1-n-terminal-western-blot.jpg)
Western blot - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Lanes 1 & 4 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/100 dilution
Lanes 2 & 5 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilution
Lanes 3 & 6 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/1000 dilution
Lanes 1-3 : HeLa cell extract (5x10e4 cells)
Lanes 4-6 : U2OS cell extract (5x10e4 cells)
Secondary
All lanes : Alexa488 goat anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 45 kDa
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![Western blot - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-196991-anti-vrk1-antibody-5d1-n-terminal-western-blot.jpg)
Western blot - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilution
Lane 1 : Extracts from TIG1 (5x10e4) cells: Luciferase RNAi (control)
Lane 2 : Extracts from TIG1 (5x10e4) cells: VRK1-1 RNAi
Lane 3 : Extracts from TIG1 (5x10e4) cells: VRK1-2 RNAi
Developed using the ECL technique.
Predicted band size: 45 kDa
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![Western blot - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-196990-anti-vrk1-antibody-5d1-n-terminal-western-blot.jpg)
Western blot - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilution
Lane 1 : Extracts from HeLa (5x10e4) cells: Luciferase RNAi (control)
Lane 2 : Extracts from HeLa (5x10e4) cells: VRK1-1 RNAi
Lane 3 : Extracts from HeLa (5x10e4) cells: VRK1-2 RNAi
Developed using the ECL technique.
Predicted band size: 45 kDa
-
![Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-196993-anti-vrk1-antibody-5d1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Immunofluorescent analysis of paraformaldehyde-fixed metaphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).
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![Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-197090-anti-vrk1-antibody-5d1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Immunofluorescent analysis of methanol-fixed interphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).
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![Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-197091-anti-vrk1-antibody-5d1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Immunofluorescent analysis of methanol-fixed metaphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).
Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.
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![Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-197092-anti-vrk1-antibody-5d1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Immunofluorescent analysis of formaldehyde-fixed interphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).
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![Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-197093-anti-vrk1-antibody-5d1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Immunofluorescent analysis of formaldehyde-fixed metaphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).
Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.
-
![Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-197094-anti-vrk1-antibody-5d1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Immunofluorescent analysis of methanol-fixed interphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).
-
![Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)](https://www.abcam.com/ps/products/171/ab171933/Images/ab171933-197095-anti-vrk1-antibody-5d1-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (ab171933)Immunofluorescent analysis of methanol-fixed metaphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).
Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.
