Vector carries no resistance gene for plant selection, streptomycin resistance for selection in bacteria and the GusPlus™ reporter gene.

The pCambia0105.1R vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with streptomycin
GRP-GusPlus™ gene for secreted GUS activity

Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.

This vector is useful for generating transformants without using a selectable marker gene.

Concentration of Antibiotics for selection.
In bacterial system:

Spectinomycin/streptomycin resistance (aadA/aminoglycoside-3’’-adenylyltransferase) in bacteria: use 50μg/mL in E.coli and A.tumefaciens.

In plant system:

No plant selection marker.