pCambia0105.1R Plant Expression Vector (ab275777)
Overview
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Product name
pCambia0105.1R Plant Expression Vector -
General notes
Vector carries no resistance gene for plant selection, streptomycin resistance for selection in bacteria and the GusPlus™ reporter gene.
The pCambia0105.1R vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with streptomycin
GRP-GusPlus™ gene for secreted GUS activityReporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.
This vector is useful for generating transformants without using a selectable marker gene.
Concentration of Antibiotics for selection.
In bacterial system:Spectinomycin/streptomycin resistance (aadA/aminoglycoside-3’’-adenylyltransferase) in bacteria: use 50μg/mL in E.coli and A.tumefaciens.
In plant system:
No plant selection marker.
Properties
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Form
Lyophilized -
Storage instructions
Shipped at 4°C. Store at -20°C. -
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