Anti-p53 antibody [9.1] (ab77813)
![Anti-p53 antibody [9.1] (ab77813)](https://www.abcam.com/ps/products/77/ab77813/Images/ab77813-457117-anti-p53-antibody-western-blot-lysate-zebrafish.jpg "Anti-p53 antibody [9.1] (ab77813)")
Key features and details
- Mouse monoclonal [9.1] to p53
- Suitable for: WB
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-p53 antibody [9.1]
See all p53 primary antibodies -
Description
Mouse monoclonal [9.1] to p53 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant full length protein corresponding to Zebrafish p53.
Database link: P79734 -
Positive control
- This antibody gave a positive signal in Roscovitine-Treated Zebrafish Embryo Lysates.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
9.1 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-p53 antibody [9.1] (ab77813)All lanes : Anti-p53 antibody [9.1] (ab77813) at 5 µg/ml
Lane 1 : Untreated Zebrafish Lysate
Lane 2 : Roscovitine-treated Zebrafish Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 20 minutesWestern blot analysis using a MOPS gel and 3% milk blocking buffer.
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![Western blot - Anti-p53 antibody [9.1] (ab77813)](https://www.abcam.com/ps/products/77/ab77813/Images/p53-Primary-antibodies-ab77813-11.jpg)
Western blot - Anti-p53 antibody [9.1] (ab77813)All lanes : Anti-p53 antibody [9.1] (ab77813) at 5 µg/ml
Lane 1 : Untreated Zebrafish Lysate
Lane 2 : Roscovitine-treated Zebrafish Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 20 kDa, 55 kDa, 57 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
