Anti-mGluR1a antibody [EPR13540] (ab183712)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13540] to mGluR1a
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-mGluR1a antibody [EPR13540]
See all mGluR1a primary antibodies -
Description
Rabbit monoclonal [EPR13540] to mGluR1a -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human cerebellum, Mouse brain and Rat brain lysates.
-
General notes
This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13540 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-mGluR1a antibody [EPR13540] (ab183712) at 1/1000 dilution
Lane 1 : Human cerebellum lysates boiled
Lane 2 : Mouse brain lysates boiled
Lane 3 : Rat brain lysates boiled
Lane 4 : Human cerebellum lysates unboiled
Lane 5 : Mouse brain lysates unboiled
Lane 6 : Rat brain lysates unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 132 kDa
Observed band size: 101,132 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in PMID: 30375476 and PMID: 11672421.
We recommend not to boil the samples after lysis to get desired WB results. -
Anti-mGluR1a antibody [EPR13540] (ab183712) at 1/5000 dilution + Human cerebellum lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 132 kDa
Observed band size: 101,200 kDa why is the actual band size different from the predicted?
-