Anti-mGluR1a antibody (ab51314)
Key features and details
- Rabbit polyclonal to mGluR1a
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-mGluR1a antibody
See all mGluR1a primary antibodies -
Description
Rabbit polyclonal to mGluR1a -
Host species
Rabbit -
Specificity
IHC-P results for Mouse have been shown to be negative for this antibody. Although, there is significant homology with Mouse, it is not predicted species. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cynomolgus monkey
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Immunogen
Synthetic peptide corresponding to Rat mGluR1a aa 1159-1171.
Sequence:SSVPSSPVSESVL
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General notes
This product was previously labelled as Metabotropic Glutamate Receptor 1, Metabotropic Glutamate Receptor 1a
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-mGluR1a antibody (ab51314)
mGluR1a staining in rat brain (singulate cortex) using ab51314 at 1/100 dilution. mGluR1a detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color).
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Western blot - Anti-mGluR1a antibody (ab51314)Anti-mGluR1a antibody (ab51314) at 1/1000 dilution + Human brain lysate at 20 µg
Secondary
AP-conjugated secondary at 1/5000 dilution
Predicted band size: 133 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
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Western blot - Anti-mGluR1a antibody (ab51314)All lanes : Anti-mGluR1a antibody (ab51314)
Lane 1 : Rat cerebellar membranes
Lane 2 : Rat cerebellar membranes + DTT
Predicted band size: 133 kDa
Observed band size: 133 kDa
