Anti-Thyroid Peroxidase ELISA Kit (ab178632)
Key features and details
- Sensitivity: 0.5 U/ml
- Range: 5 U/ml - 320 U/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Anti-Thyroid Peroxidase ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos. Serum 20 3.8% Pos. Serum 20 4.8% Neg. Serum 20 6% Inter-assay Sample n Mean SD CV% Pos. Serum 12 113.3U/ml 7.1% Pos. Serum 12 176U/ml 5.7% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
Range
5 U/ml - 320 U/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s anti-Thyroid Peroxidase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroid peroxidase in Human serum or plasma (citrate).
A 96-well plate has been precoated with recombinant thyroid peroxidase to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroid peroxidase-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroid peroxidase sample captured in plate.
Platform
MicroplateProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit Thyroid peroxidase Anti-IgG HRP Conjugate 1 x 20ml Thyroid peroxidase Coated Microplate (12 x 8 wells) 1 unit Thyroid peroxidase Negative Control 1 x 2ml Thyroid peroxidase Positive Control 1 x 2ml Thyroid peroxidase Standard A - 0 U/mL 1 x 2ml Thyroid peroxidase Standard B - 5 U/mL 1 x 2ml Thyroid peroxidase Standard C - 10 U/mL 1 x 2ml Thyroid peroxidase Standard D - 20 U/mL 1 x 2ml Thyroid peroxidase Standard E - 80 U/mL 1 x 2ml Thyroid peroxidase Standard F - 320 U/mL 1 x 2ml TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit Thyroid peroxidase Anti-IgG HRP Conjugate 1 x 20ml Thyroid peroxidase Coated Microplate (12 x 8 wells) 1 unit Thyroid peroxidase Negative Control 1 x 2ml Thyroid peroxidase Positive Control 1 x 2ml Thyroid peroxidase Standard A - 0 U/mL 1 x 2ml Thyroid peroxidase Standard B - 5 U/mL 1 x 2ml Thyroid peroxidase Standard C - 10 U/mL 1 x 2ml Thyroid peroxidase Standard D - 20 U/mL 1 x 2ml Thyroid peroxidase Standard E - 80 U/mL 1 x 2ml Thyroid peroxidase Standard F - 320 U/mL 1 x 2ml TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative standard curve using ab178632