Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NFYA antibody [EPR9061] (ab139402)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9061] to NFYA
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-NFYA antibody [EPR9061]
    See all NFYA primary antibodies

  • Description

    Rabbit monoclonal [EPR9061] to NFYA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NFYA aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Recombinant Human NFYA protein (ab131777) can be used as a positive control in WB. NIH 3T3, HeLa, 293T, and Jurkat cell lysates; NIH 3T3 cells. This antibody gave a positive result when used in the following methanol fixed cell lines: Malme-3M

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9061

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NFYA antibody [EPR9061] (ab139402)
    Western blot - Anti-NFYA antibody [EPR9061] (ab139402)
    All lanes : Anti-NFYA antibody [EPR9061] (ab139402) at 1/1000 dilution

    Lane 1 : NIH 3T3 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] (ab139402)
    Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] (ab139402)

    ab139402 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab139402 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry - Anti-NFYA antibody [EPR9061] (ab139402)
    Flow Cytometry - Anti-NFYA antibody [EPR9061] (ab139402)
    Flow Cytometrical analysis of permeabilized NIH 3T3 cells labelling NFYA with ab139402 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
  • Anti-NFYA antibody [EPR9061] (ab139402)
    Anti-NFYA antibody [EPR9061] (ab139402)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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