Anti-Carboxypeptidase B/CPB antibody (ab153716)
Key features and details
- Rabbit polyclonal to Carboxypeptidase B/CPB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Carboxypeptidase B/CPB antibody
See all Carboxypeptidase B/CPB primary antibodies -
Description
Rabbit polyclonal to Carboxypeptidase B/CPB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Pig -
Immunogen
Recombinant fragment corresponding to Human Carboxypeptidase B/CPB aa 144-417.
Database link: P15086 -
Positive control
- WB: A549, HeLa, AsPC-1, HepG2, transfected 293T and HCT116 whole cell lysates, mouse and rat tissue lysate; IHC-P: Human Ca922 xenograft, mouse and rat pancreas tissue.
-
General notes
This product was previously labelled as Carboxypeptidase B
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/1000 dilution. Antigen Retrieval performed using citrate buffer, pH 6.0, 15 min
-
All lanes : Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : AsPC-1 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa
-
All lanes : Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/5000 dilution
Lane 1 : Non-transfected 293T cell lysate
Lane 2 : Carboxypeptidase B/CPB transfected 293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa
-
Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/50000 dilution + Mouse pancreas tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa
-
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/1000 dilution. Antigen Retrieval performed using citrate buffer, pH 6.0, 15 min
-
Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/50000 dilution + Rat pancreas tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa
-
Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/1000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human Ca922 xenograft tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/250 dilution.