Anti-HMGCL antibody [9F5AB9] (ab110295)
![Anti-HMGCL antibody [9F5AB9] (ab110295)](https://www.abcam.com/ps/products/110/ab110295/Images/HMGCL-Primary-antibodies-ab110295-1.jpg "Anti-HMGCL antibody [9F5AB9] (ab110295)")
Key features and details
- Mouse monoclonal [9F5AB9] to HMGCL
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-HMGCL antibody [9F5AB9]
See all HMGCL primary antibodies -
Description
Mouse monoclonal [9F5AB9] to HMGCL -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type. This information is considered to be commercially sensitive.
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Positive control
- HeLa cells and human liver mitochondria.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
9F5AB9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HMGCL antibody [9F5AB9] (ab110295)Immunocytochemistry image of ab110295 stained Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110295 at 5 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates mainly in mitochondria.
