Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (ab250789)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15303] to NDUFA8 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free
    See all NDUFA8 primary antibodies

  • Description

    Rabbit monoclonal [EPR15303] to NDUFA8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250789 is the carrier-free version of ab184952. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250789 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (ab250789)
    Western blot - Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (ab250789)
    All lanes : Anti-NDUFA8 antibody [EPR15303] (ab184952) at 1/20000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal muscle lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab184952, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (ab250789)
    Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (ab250789)

    This data was developed using ab184952, the same antibody clone in a different buffer formulation.

    Western blot analysis of NDUFA8 in Human fetal heart lysate immunoprecipitated with ab184952 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal heart lysate.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (ab250789)
    Anti-NDUFA8 antibody [EPR15303] - BSA and Azide free (ab250789)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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