Anti-Nav1.8/SCN10A antibody (ab139510)
Key features and details
- Rabbit polyclonal to Nav1.8/SCN10A
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Nav1.8/SCN10A antibody
See all Nav1.8/SCN10A primary antibodies -
Description
Rabbit polyclonal to Nav1.8/SCN10A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide corresponding to Mouse Nav1.8/SCN10A aa 1850-1950 conjugated to keyhole limpet haemocyanin.
Database link: Q6QIY3 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Rat Cerebellum.
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General notes
This product was previously labelled as Nav1.8
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 260 kDa (predicted molecular weight: 221 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 260 kDa (predicted molecular weight: 221 kDa).Target
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Function
Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms. -
Tissue specificity
Expressed in the dorsal root ganglia and sciatic nerve. -
Involvement in disease
Episodic pain syndrome, familial, 2 -
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Contains 1 IQ domain. -
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsUbiquitinated by NEDD4L; which promotes its endocytosis.
Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents. -
Cellular localization
Cell membrane. It can be translocated to the cell membrane through association with S100A10. - Information by UniProt
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Database links
- Entrez Gene: 20264 Mouse
- Entrez Gene: 29571 Rat
- SwissProt: P70276 Mouse
- SwissProt: Q6QIY3 Mouse
- SwissProt: Q62968 Rat
- Unigene: 247042 Mouse
- Unigene: 455125 Mouse
- Unigene: 489505 Mouse
see all -
Alternative names
- hPN3 antibody
- mPN3 antibody
- Peripheral nerve sodium channel 3 antibody
see all
Images
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Western blot - Anti-Nav1.8/SCN10A antibody (ab139510)All lanes : Anti-Nav1.8/SCN10A antibody (ab139510) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 221 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesNav1.8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab139510 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab139510 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Nav1.8/SCN10A antibody (ab139510)All lanes : Anti-Nav1.8/SCN10A antibody (ab139510) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 221 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesNav1.8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab139510 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
