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Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)

Key features and details

  • Mouse monoclonal [N134/12] to Nav1.8/SCN10A
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human, Monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Nav1.8/SCN10A antibody [N134/12]
    See all Nav1.8/SCN10A primary antibodies

  • Description

    Mouse monoclonal [N134/12] to Nav1.8/SCN10A

  • Host species

    Mouse

  • Specificity

    No cross reactivity against other Nav channels.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    WB
    Monkey
    See all applications and species data

  • Immunogen

    Fusion protein corresponding to Rat Nav1.8/SCN10A aa 1724-1956.
    Database link: Q62968

  • Positive control

    • WB: COS cell lysate transiently expressing Nav1.8/SCN10A. Flow Cyt: SH-SY5Y cells. IHC-P: Mouse backskin tissue.

  • General notes

    The clone number has been updated from S134-12 to N134/12, both clone numbers name the same antibody clone.

     

     This product was previously labelled as Nav1.8

     

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)

    Paraffin-embedded mouse backskin tissue stained for Nav1.8/SCN10A using ab93616 at 1/100 dilution in immunohistochemical analysis.

    Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.

  • Flow Cytometry - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Flow Cytometry - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)

    Overlay histogram showing SH-SY5Y cells stained with ab93616 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93616, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Western blot - Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616)
    Anti-Nav1.8/SCN10A antibody [N134/12] (ab93616) at 1/1000 dilution + Monkey COS cell lysate transiently expressing Nav1.8/SCN10A. at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 220 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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