Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PIN4 antibody [EPR10033] (ab155283)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10033] to PIN4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PIN4 antibody [EPR10033]
    See all PIN4 primary antibodies

  • Description

    Rabbit monoclonal [EPR10033] to PIN4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PIN4. The exact sequence is proprietary.
    Database link: Q9Y237

  • Positive control

    • 293T, Human skeletal muscle, Human fetal liver, Mouse liver, HepG2 and RAW264.7 cell lysates; Human breast and tonsil tissues. ICC/IF: HepG2 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
    Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
    All lanes : Anti-PIN4 antibody [EPR10033] (ab155283) at 1/1000 dilution

    Lane 1 : 293T lysate
    Lane 2 : Human skeletal muscle lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : HepG2 lysate
    Lane 6 : RAW264.7 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 13 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (ab155283)
    Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (ab155283)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling PIN4 with purified ab155283 at 1/150 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)

    Immunohistochemical analysis of paraffin embedded Human breast tissue, labeling PIN4 with ab155283 at a 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PIN4 with ab155283 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-PIN4 antibody [EPR10033] (ab155283)
    Anti-PIN4 antibody [EPR10033] (ab155283)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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