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Epigenetics and Nuclear Signaling DNA / RNA Translation Mito. Translation

Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)

Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16328] to MRPL48 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-MRPL48 antibody [EPR16328] - BSA and Azide free
    See all MRPL48 primary antibodies
  • Description

    Rabbit monoclonal [EPR16328] to MRPL48 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251194 is the carrier-free version of ab194826. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251194 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR16328
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Mito. Translation
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism

Images

  • Western blot - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Western blot - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    All lanes : Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab194826, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)

    This data was developed using ab194826, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling MRPL48 with ab194826 at 1/150 dilution. A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194826. Cytoplasm staining on human cervix carcinoma tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Immunocytochemistry - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    This data was developed using ab194826, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A431 cells labeling MRPL48 with ab194826 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HepG2 cell line was observed.
  • Western blot - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Western blot - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/2000 dilution + Human fetal heart tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab194826, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Flow Cytometry - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    This data was developed using ab194826, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labelling MRPL48 (red) with purified ab194826 at dilution of 1/60. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Western blot - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Western blot - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    All lanes : Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab194826, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Immunoprecipitation - Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    This data was developed using ab194826, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab194826 at 1/30 dilution (lane 1) or PBS control (lane 2)For Western blot: ab194826 was used at 1/1000 dilution (0.6µg/ml). Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)
    Anti-MRPL48 antibody [EPR16328] - BSA and Azide free (ab251194)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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