Anti-GFM2 antibody (ab229463)
Key features and details
- Rabbit polyclonal to GFM2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GFM2 antibody
See all GFM2 primary antibodies -
Description
Rabbit polyclonal to GFM2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human GFM2 (internal sequence). The exact sequence is proprietary.
Database link: Q969S9 -
Positive control
- WB: HEK-293T and HepG2 whole cell extract. ICC/IF: HeLa cells. IHC-P: Mouse testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM2 antibody (ab229463)
Paraffin-embedded mouse testis tissue stained for GFM2 using ab229463 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-GFM2 antibody (ab229463)All lanes : Anti-GFM2 antibody (ab229463) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-GFM2 antibody (ab229463)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GFM2 (green) using ab229463 at 1/500 dilution in ICC/IF. Cells were fixed with 2% paraformaldehyde/culture medium at 37oC for 30 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
