Salicylate Assay Kit (Colorimetric) (ab273321)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: EDTA Plasma, Hep Plasma, Serum
- Sensitivity: 0.19 mM
Overview
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Product name
Salicylate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Serum, Hep Plasma, EDTA Plasma -
Assay type
Quantitative -
Sensitivity
0.19 mM -
Range
0.2 mM - 4 mM -
Assay duration
Multiple steps standard assay -
Product overview
Salicylate Assay Kit (Colorimetric) (ab273321) is a high-throughput adaptable microplate-based assay that allows for rapid quantification of salicylate levels in biological fluids. In the assay, salicylate is enzymatically metabolized to catechol, with concomitant oxidation of a cofactor, resulting in a decrease in absorbance at OD = 405 nm that is proportional to the concentration of salicylate present.
The assay is not affected by other NSAIDs and has a limit of detection of 0.19 mM with a reliable linear range from 1 – 20 nmole salicylate per well (corresponding to 0.2 – 4 mM salicylate in undiluted samples).
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cofactor Solution (Lyophilized) 1 vial Matrix Replicator 1 x 500µl Salicylate Assay Buffer 1 x 25ml Salicylate Enzyme Mix (Lyophilized) 1 vial Salicylate Standard (Lyophilized) 1 vial -
Research areas
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Relevance
Salicylates are a group of chemical substances with anti-inflammatory properties which includes aspirin, choline salicylate, magnesium salicylate, sodium salicylate and salsalate. It can also be found in its natural form in vegetables, fruit, herbs and plants.
Images
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Salicylate standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Typical Salicylate standard curve.
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Estimation of Salicylate in human serumExample data.
Normal (drug-free) 'off-the-clot' pooled serum was split into aliquots and spiked with either 0.5 mM, 1 mM, 2 mM or 3 mM Salicylate (5 µL of serum was assayed in all cases). Mean Salicylate concentrations detected in spiked serum were 0.51 mM, 0.98 mM, 1.98 mM and 2.91 mM, respectively (mean spike recovery across all concentrations was 97-102%). In the 6 mM sample, pooled human serum spiked with 6 mM Salicylate was diluted at a 1:1 ration with the included Matrix Replicator and assayed (5 µL of pre-diluted serum). Salicylate concentration detected in the diluted sample was 2.93 mM x 2 = 5.85 mM (spike recovery of 97.6%). Data are mean + SEM of 3 replicates, assayed according to the kit protocol.