Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MBIP antibody [EPR13951] (ab186750)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13951] to MBIP
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MBIP antibody [EPR13951]
    See all MBIP primary antibodies

  • Description

    Rabbit monoclonal [EPR13951] to MBIP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela and 293 cell lysates, Human fetal lung and Human fetal heart lysates; Human cardiac muscle and Human clear cell carcinoma of kidney tissues; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13951

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
    Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
    All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa



    Blocking buffer:  5% NFDM/TBST

  • Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
    Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
    All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution

    Lane 1 : Human fetal lung lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 39 kDa



    Blocking buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MBIP with ab186750 at 1/250 dilution.  Secondary antibody: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)

    Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling MBIP with ab186750 at 1/250 dilution. Secondary ab: ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MBIP antibody [EPR13951] (ab186750)
    Immunocytochemistry/ Immunofluorescence - Anti-MBIP antibody [EPR13951] (ab186750)

    Immunofluorescence analysis of HeLa cells labeling MBIP with ab186750 at 1/500 dilution.  Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488).  Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue).

  • Flow Cytometry - Anti-MBIP antibody [EPR13951] (ab186750)
    Flow Cytometry - Anti-MBIP antibody [EPR13951] (ab186750)

    Flow cytometric analysis of HeLa cell labeling MBIP using ab186750 at 1/400 dilution. Secondary antibody: Goat anti rabbit IgG (FITC) at 1/400 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.

  • Anti-MBIP antibody [EPR13951] (ab186750)
    Anti-MBIP antibody [EPR13951] (ab186750)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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