Anti-MBIP antibody [EPR13951] (ab186750)
![Anti-MBIP antibody [EPR13951] (ab186750)](https://www.abcam.com/ps/products/186/ab186750/Images/ab186750-219988-anti-map3k12-binding-inhibitory-protein-1-antibody-epr13951-western-blot.jpg "Anti-MBIP antibody [EPR13951] (ab186750)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13951] to MBIP
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-MBIP antibody [EPR13951]
See all MBIP primary antibodies -
Description
Rabbit monoclonal [EPR13951] to MBIP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela and 293 cell lysates, Human fetal lung and Human fetal heart lysates; Human cardiac muscle and Human clear cell carcinoma of kidney tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13951 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MBIP antibody [EPR13951] (ab186750)All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDaBlocking buffer: 5% NFDM/TBST
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![Western blot - Anti-MBIP antibody [EPR13951] (ab186750)](https://www.abcam.com/ps/products/186/ab186750/Images/ab186750-219989-anti-map3k12-binding-inhibitory-protein-1-antibody-epr13951-western-blot.jpg)
Western blot - Anti-MBIP antibody [EPR13951] (ab186750)All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 39 kDaBlocking buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)](https://www.abcam.com/ps/products/186/ab186750/Images/ab186750-219990-anti-map3k12-binding-inhibitory-protein-1-antibody-epr13951-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MBIP with ab186750 at 1/250 dilution. Secondary antibody: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)](https://www.abcam.com/ps/products/186/ab186750/Images/ab186750-219992-anti-map3k12-binding-inhibitory-protein-1-antibody-epr13951-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling MBIP with ab186750 at 1/250 dilution. Secondary ab: ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-MBIP antibody [EPR13951] (ab186750)](https://www.abcam.com/ps/products/186/ab186750/Images/ab186750-219994-anti-map3k12-binding-inhibitory-protein-1-antibody-epr13951-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MBIP antibody [EPR13951] (ab186750)Immunofluorescence analysis of HeLa cells labeling MBIP with ab186750 at 1/500 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488). Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue).
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![Flow Cytometry - Anti-MBIP antibody [EPR13951] (ab186750)](https://www.abcam.com/ps/products/186/ab186750/Images/ab186750-219996-anti-map3k12-binding-inhibitory-protein-1-antibody-epr13951-flow-cytometry.jpg)
Flow Cytometry - Anti-MBIP antibody [EPR13951] (ab186750)Flow cytometric analysis of HeLa cell labeling MBIP using ab186750 at 1/400 dilution. Secondary antibody: Goat anti rabbit IgG (FITC) at 1/400 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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![Anti-MBIP antibody [EPR13951] (ab186750)](https://www.abcam.com/ps/products/186/ab186750/Images/ab186750-7-benefits-of-recombinant-antibodies.png)
Anti-MBIP antibody [EPR13951] (ab186750)
