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Anti-MAGEA4 antibody [OTI1F9] (ab139297)

Key features and details

  • Mouse monoclonal [OTI1F9] to MAGEA4
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-MAGEA4 antibody [OTI1F9]
    See all MAGEA4 primary antibodies

  • Description

    Mouse monoclonal [OTI1F9] to MAGEA4

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human MAGEA4 aa 1-317. Produced in HEK-293T cells (NP_001011550).
    Sequence:

    MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGT LEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSP DAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPV IFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKT GLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQD WVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRI AYPSLREAALLEEEEGV


    Database link: P43358
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human bladder carcinoma tissue. WB: HEK-293T cell lysate transfected with pCMV6-ENTRY MAGEA4 cDNA.

  • General notes

    Clone OTI1F9 (formerly 1F9).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Clonality

    Monoclonal

  • Clone number

    OTI1F9

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [OTI1F9] (ab139297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [OTI1F9] (ab139297)

    Paraffin-embedded human bladder carcinoma tissue stained for MAGEA4 using ab139297 at 1/150 dilution in immunohistochemical analysis.

  • Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297)
    Western blot - Anti-MAGEA4 antibody [OTI1F9] (ab139297)
    All lanes : Anti-MAGEA4 antibody [OTI1F9] (ab139297) at 1/4000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY MAGEA4 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 35 kDa

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