Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4240] to GPA33 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GPA33 antibody [EPR4240] - BSA and Azide free
    See all GPA33 primary antibodies

  • Description

    Rabbit monoclonal [EPR4240] to GPA33 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human small intestine, colon and fetal colon tissue lysates; HT-29 and MOLT-4 cell lysates; IHC-P: Human colon tissue. Human colon cancer tissue. Human ovarian cancer tissue; IP: HT-29 cell lysate.

  • General notes

    ab247720 is the carrier-free version of ab108938 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247720 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)
    Western blot - Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)
    All lanes : Anti-GPA33 antibody [EPR4240] (ab108938) at 1/1000 dilution (Purified)

    Lane 1 : Human colon lysates
    Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 36 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108938).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling GPA33 with Purified ab108938 at 1:400 dilution (0.25 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108938).

  • Immunoprecipitation - Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)
    Immunoprecipitation - Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)

    Purified ab108938 at 1:20 dilution (0.5ug) immunoprecipitating GPA33 in HT-29 whole cell lysate.

    Lane 1 (input): HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate (10µg)

    Lane 2 (+): ab108938 + HT-29 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108938 in HT-29 whole cell lysate.

    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.

    Blocking Buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

    Observed band size: 50 kDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108938).

  • Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)
    Anti-GPA33 antibody [EPR4240] - BSA and Azide free (ab247720)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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