Anti-MAGEA4 antibody (ab229011)
Key features and details
- Rabbit polyclonal to MAGEA4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAGEA4 antibody
See all MAGEA4 primary antibodies -
Description
Rabbit polyclonal to MAGEA4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant fragment within Human MAGEA4 (internal sequence). The exact sequence is proprietary.
Database link: P43358 -
Positive control
- WB: A549 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: A-375 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody (ab229011)
Paraffin-embedded human breast cancer tissue stained for MAGEA4 using ab229011 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-MAGEA4 antibody (ab229011)Anti-THEM2 antibody (ab228011) at 1/2000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 35 kDa12% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-MAGEA4 antibody (ab229011)4% paraformaldehyde-fixed A-375 (human malignant melanoma cell line) cells stained for MAGEA4 (green) using ab229011 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
