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Cancer Tumor immunology Tumor-associated antigens

Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)

Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16258] to MAGEA9 - BSA and Azide free
  • Suitable for: IP, WB, Flow Cyt (Intra)
  • Reacts with: Human

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Overview

  • Product name

    Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free
    See all MAGEA9 primary antibodies
  • Description

    Rabbit monoclonal [EPR16258] to MAGEA9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251089 is the carrier-free version of ab191568.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR16258
  • Isotype

    IgG
  • Research areas

    • Cancer
    • Tumor immunology
    • Tumor-associated antigens

Images

  • Immunoprecipitation - Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    Immunoprecipitation - Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    This data was developed using ab191568, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of human testis tissue lysate labeling MAGEA9 using ab191568 at 1/100 dilution (Left Lane). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Right Lane: PBS instead of human testis tissue lysate.
  • Western blot - Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    Western blot - Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution + K562 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa



    This data was developed using ab191568, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    Flow Cytometry - Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    This data was developed using ab191568, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 562 cells labeling MAGEA9 using ab191568 at 1/350 dilution (red) or rabbit monoclonal IgG as isotype control (green). Secondary antibody Goat anti rabbit IgG (FITC). Cells were fixed in 2% paraformaldehyde.
  • Western blot - Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    Western blot - Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa



    This data was developed using ab191568, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)
    Anti-MAGEA9 antibody [EPR16258] - BSA and Azide free (ab251089)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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