Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (ab213598)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12733] to LOXL2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LOXL2 antibody [EPR12733] - BSA and Azide free
    See all LOXL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12733] to LOXL2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Our lab has been unable to use this product successfully in IHC-P and so we cannot guarantee that it will work in this application. However, some customers have been successful with this antibody in IHC-P.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MDA-MB-435, U87-MG, and MCF7 cell lysates.

  • General notes

    Ab213598 is the carrier-free version of ab179810. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab213598 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (ab213598)
    Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (ab213598)
    All lanes : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LOXL2 knockout HeLa cell lysate
    Lane 3 : HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate
    Lane 4 : Untreated HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 87 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab179810).

    Lanes 1-4: Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264807 (knockout cell lysate ab257166) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (ab213598)
    Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (ab213598)
    All lanes : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LOXL2 knockout HeLa cell lysate
    Lane 3 : HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate
    Lane 4 : Untreated HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 87 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab179810).

    Lanes 1-4: Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261804 (knockout cell lysate ab257168) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (ab213598)
    Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (ab213598)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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