Anti-LOXL2 antibody [EPR23035-105] (ab260046)
![Anti-LOXL2 antibody [EPR23035-105] (ab260046)](https://www.abcam.com/ps/products/260/ab260046/Images/ab260046-3-anti-loxl2-antibody-epr23035-105-western-blot-hela-human.jpg "Anti-LOXL2 antibody [EPR23035-105] (ab260046)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23035-105] to LOXL2
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-LOXL2 antibody [EPR23035-105]
See all LOXL2 primary antibodies -
Description
Rabbit monoclonal [EPR23035-105] to LOXL2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-435S, MDA-MB-231, U-87 MG, untreated HeLa and HeLa (treated with 2mM for 24 hours) whole lysates. IP: MDA-MB-435S whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23035-105 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LOXL2 antibody [EPR23035-105] (ab260046)All lanes : Anti-LOXL2 antibody [EPR23035-105] (ab260046) at 1/1000 dilution
Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa treated with 2mM Deferoxamine mesylate (DFO) for 24 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 87 kDa
Observed band size: 100,70 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
LOXL2 is a glycoprotein. Unprocessed LOXL2 (100kDa) and processed LOXL2(70kDa) are observed.
The expression of LOXL2 is elevated under hypoxia condition.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:20026874; PMID: 27770022).
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![Western blot - Anti-LOXL2 antibody [EPR23035-105] (ab260046)](https://www.abcam.com/ps/products/260/ab260046/Images/ab260046-2-anti-loxl2-antibody-epr23035-105-western-blot-human.jpg)
Western blot - Anti-LOXL2 antibody [EPR23035-105] (ab260046)All lanes : Anti-LOXL2 antibody [EPR23035-105] (ab260046) at 1/1000 dilution
Lane 1 : MDA-MB-435S (human mammary gland ductal carcinoma melanocyte) whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 87 kDa
Observed band size: 100,70 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking and dilution buffer: 5% NFDM/TBST.
LOXL2 is a glycoprotein. Unprocessed LOXL2 (100kDa) and processed LOXL2(70kDa) are observed.
The molecular weight observed is consistent with what has been described in the literature (PMID: 27770022).
Negative control: MCF7 (PMID: 12154058).
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![Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] (ab260046)](https://www.abcam.com/ps/products/260/ab260046/Images/ab260046-1-anti-loxl2-antibody-epr23035-105-immunoprecipitation-mdamb435s-human.jpg)
Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] (ab260046)LOXL2 was immunoprecipitated from 0.35 mg MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate with ab260046 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260046 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate 10ug
Lane 2: ab260046 IP in MDA-MB-435S whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260046 in MDA-MB-435S whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
LOXL2 is a glycoprotein. Unprocessed LOXL2 (100kDa) and processed LOXL2(70kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 27770022).
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![Anti-LOXL2 antibody [EPR23035-105] (ab260046)](https://www.abcam.com/ps/products/260/ab260046/Images/ab260046-4-benefits-of-recombinant-antibodies.png)
Anti-LOXL2 antibody [EPR23035-105] (ab260046)
