Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)
![Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)](https://www.abcam.com/ps/products/267/ab267495/Images/ab267495-1-anti-loxl2-antibody-epr23035-105-immunoprecipitation-mdamb435s-human.jpg "Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23035-105] to LOXL2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free
See all LOXL2 primary antibodies -
Description
Rabbit monoclonal [EPR23035-105] to LOXL2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-435S, MDA-MB-231, U-87 MG, untreated HeLa and HeLa (treated with 2mM for 24 hours) whole lysates. IP: MDA-MB-435S whole cell lysate.
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General notes
ab267495 is the carrier-free version of ab260046. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23035-105 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)
LOXL2 was immunoprecipitated from 0.35 mg MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate with ab260046 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260046 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate 10ug
Lane 2: ab260046 IP in MDA-MB-435S whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260046 in MDA-MB-435S whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
LOXL2 is a glycoprotein. Unprocessed LOXL2 (100kDa) and processed LOXL2(70kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 27770022).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260046).
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![Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)](https://www.abcam.com/ps/products/267/ab267495/Images/ab267495-2-benefits-of-recombinant-antibodies.png)
Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)
