Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23035-105] to LOXL2 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free
    See all LOXL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23035-105] to LOXL2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-435S, MDA-MB-231, U-87 MG, untreated HeLa and HeLa (treated with 2mM for 24 hours) whole lysates. IP: MDA-MB-435S whole cell lysate.

  • General notes

    ab267495 is the carrier-free version of ab260046. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)
    Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)

    LOXL2 was immunoprecipitated from 0.35 mg MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate with ab260046 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260046 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate 10ug

    Lane 2: ab260046 IP in MDA-MB-435S whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260046 in MDA-MB-435S whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    LOXL2 is a glycoprotein. Unprocessed LOXL2 (100kDa) and processed LOXL2(70kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 27770022).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260046).

  • Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)
    Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (ab267495)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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