Human LOXL2 ELISA Kit (ab275893)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24.98 pg/ml
- Range: 117.19 pg/ml - 7500 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human LOXL2 ELISA Kit
See all LOXL2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4.7% Inter-assay Sample n Mean SD CV% Extract 3 5.5% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
24.98 pg/ml -
Range
117.19 pg/ml - 7500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 113 103% - 122% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human LOXL2 ELISA kit (ab275893) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LOXL2 protein in human cell extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LOXL2 with 24.98 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
LOXL2 is a member of the lysyl oxidase (LOX) family of copper dependent amine oxidases. The LOX family are involved in crosslinking, resulting in increased tissue stiffness. LOXL2 is involved in histone modification and in regulating proteins involved in transcription. LOXL2 implicated in several types of cancer, where its upregulation is associated with a poor prognosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human LOXL2 Capture Antibody 1 x 600µl 10X Human LOXL2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human LOXL2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Tissue specificity
Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate. -
Sequence similarities
Belongs to the lysyl oxidase family.
Contains 4 SRCR domains. -
Post-translational
modificationsThe lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
-
Alternative names
- LOR 2
- LOR2
- LOX L2
see all -
Database links
- Entrez Gene: 4017 Human
- Omim: 606663 Human
- SwissProt: Q9Y4K0 Human
- Unigene: 626637 Human
- Unigene: 661130 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human LOXL2 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The LOXL2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native LOXL2 in human U-87 MG extract based on a 50 µg/mL extract load.The concentrations of LOXL2 were measured in duplicate and interpolated from the LOXL2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LOXL2 concentration was determined to be 5,843.79 pg/ml in U-87 MG extract.
-
Sandwich ELISA - Human LOXL2 ELISA Kit (ab275893)To learn more about the advantages of recombinant antibodies see here.
