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Signal Transduction Cytoskeleton / ECM Extracellular Matrix

Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3190(2)] to Laminin beta 1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free
    See all Laminin beta 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3190(2)] to Laminin beta 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney, HeLa and A431 lysates. Flow Cyt: A431 cells
  • General notes

    Ab247822 is the carrier-free version of ab109293. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247822 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3190(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina

Images

  • Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)
    Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)
    All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LAMB1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 198 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab109293).

    Lanes 1- 2: Merged signal (red and green). Green - ab109293 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

     ab109293 was shown to react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type HeLa and LAMB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109293 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)
    Flow Cytometry - Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)
    This data was developed using ab109293, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized A431 cells using ab109293 (red) at a diluiton of 1/100 or a rabbit IgG (negative) (green).
  • Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)
    Western blot - Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)
    All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 198 kDa



    This data was developed using ab109293, the same antibody clone in a different buffer formulation.

  • Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)
    Anti-Laminin beta 1 antibody [EPR3190(2)] - BSA and Azide free (ab247822)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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