Apamin, blocker of small conductance Ca2+-activated K+ channels (ab120268)
Key features and details
- Blocker of small conductance Ca2+-activated K+ channels
- CAS Number: 24345-16-2
- Purity: > 95%
- Soluble in water
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Apamin, blocker of small conductance Ca2+-activated K+ channels -
Description
Blocker of small conductance Ca2+-activated K+ channels -
Alternative names
- SK channel
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Biological description
Peptide neurotoxin that naturally occurs in Apis mellifera bee venom. Blocks small conductance Ca2+ activated K+ channels (SK). Selective for KCa2.1-2.3 (SK1-3) isoforms. Blocks hSK1, rSK2 and liver rSK3 (IC50 values are 8 nM, 83 pM and 0.6 nM, respectively).
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Purity
> 95% -
CAS Number
24345-16-2 -
Chemical structure
Properties
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Molecular weight
2027.35 -
Molecular formula
C79H131N31O24S4 -
Sequence
CNCKAPETALCARRCQQH (Modifications: C-terminal amide; Disulfide bonds: 1-11, 3-15) -
PubChem identifier
16218850 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Immunohistochemistry - Apamin, blocker of small conductance Ca2+-activated K+ channels (ab120268) Image from Bertran-Gonzalez J et al., PloS one., 7(12): e53195. Fig 6B.; doi: 10.1371/journal.pone.0053195 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Confocal images from a transcardially-fixed rat brain previously injected with blockers (left-side dorsal striatum, Left DMS) and blockers plus apamin (right-side dorsal striatum, Right DMS) showing double staining for ChAT and p-Ser240–244-S6rp. Top panels are low-magnification images showing several cholinergic interneurons in the same focal plane. Bottom panels are higher magnification images showing p-S6rp signal intensity in CINs from the left (blockers) and right (blockers + apamin) striata of the same animal. Insets show corresponding ChAT staining.
Bertran-Gonzalez J et al., PloS one., 7(12): e53195. Fig 6B.; doi: 10.1371/journal.pone.0053195