Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (ab279712)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24131-79] to Monoamine Oxidase B/MAOB - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free
    See all Monoamine Oxidase B/MAOB primary antibodies

  • Description

    Rabbit monoclonal [EPR24131-79] to Monoamine Oxidase B/MAOB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt (Intra),ICC or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and liver tissue lysates; Rat brain and liver tissue lysate; E.coli extracts containing his-tagged mouse Amine oxidase [flavin-containing] B recombinant protein (aa 2-489). IHC-P: Mouse liver; Rat liver. IP: Mouse liver tissue lysate.

  • General notes

    ab279712 is the carrier-free version of ab259928. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab279712 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (ab279712)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (ab279712)
    All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    This data was developed using ab259928, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 8058968).

    Low expression: mouse testis.

    Protein aggregates may be caused by boiling of samples .

     Exposure time: 15 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab279712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab279712)

    This data was developed using ab259928, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on mouse liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (ab279712)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] - BSA and Azide free (ab279712)
    All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928) at 1/1000 dilution

    Lane 1 : E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi B recombinant protein (aa 2-489) at 10 µl
    Lane 2 : E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi A recombinant protein (aa 1-497) at 4 µl

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 59 kDa



    This data was developed using ab259928, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates. 

    Exposure time: 15 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab279712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab279712)

    This data was developed using ab259928, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on rat liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - (ab279712)
    Immunoprecipitation - (ab279712)

    This data was developed using ab259928, the same antibody clone in a different buffer formulation.

    Monoamine Oxidase B was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259928 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259928 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver tissue lysate 10 ug

    Lane 2: ab259928 IP in Mouse liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259928 in mouse liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    Sample loaded onto lane 1 are non-boiled as boiling may cause protein aggregates.

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