Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (ab249882)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11854] to L3MBTL3 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free
    See all L3MBTL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11854] to L3MBTL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human L3MBTL3. The exact sequence is proprietary.
    Database link: Q96JM7

  • Positive control

    • WB: HeLa, 293T and Human fetal brain lysates.

  • General notes

    Ab249882 is the carrier-free version of ab175232. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249882 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (ab249882)
    Western blot - Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (ab249882)
    All lanes : Anti-L3MBTL3 antibody [EPR11854] (ab175232) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : L3MBTL3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 88 kDa
    Observed band size: 88 kDa



    This data was developed using ab175232, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab175232 observed at 88 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab175232 Anti-L3MBTL3 antibody [EPR11854] was shown to specifically react with L3MBTL3 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266172 (knockout cell lysate ab258489) was used. Wild-type and L3MBTL3 knockout samples were subjected to SDS-PAGE. ab175232 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (ab249882)
    Western blot - Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (ab249882)
    All lanes : Anti-L3MBTL3 antibody [EPR11854] (ab175232) at 1/1000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293T lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 88 kDa



    This data was developed using ab175232, the same antibody clone in a different buffer formulation.

     

  • Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (ab249882)
    Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (ab249882)

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