Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023) - BSA and Azide free
  • Suitable for: ELISA, Dot blot, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free
    See all JAK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1899(2)] to JAK1 (phospho Y1022 + Y1023) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, Dot blot, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos cell lysates, treated with pervanadate; Human colon adenocarcinoma tissue.

  • General notes

    ab203784 is the carrier-free version of ab138005 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab203784 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
    Dot Blot - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)

    Primary antibody: 1/1000 (unpurified)

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051)

    Secondary ab dilution: 1/100,000

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM /TBST

    Lane 1: JAK1 (pY1022) phospho peptide

    Lane 2: JAK1 (pY1023) phospho peptide

    Lane 3: JAK1 (pY1022/pY1023) phospho peptide

    Lane 4: JAK1 non-phospho peptide

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma sections labelling JAK1 (phospho Y1022 + Y1023) with purified ab138005 at dilution of 1/50. The secondary antibody used was ImmunoHistoProbe HRP Polymer for Rabbit IgG at dilution of 1:0. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).

  • ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
    ELISA - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)

    Antigen:

    1. JAK1 (pY1022) phospho peptide
    2. JAK1 (pY1023) phospho peptide
    3. JAK1 non-phospho peptide

    Antigen concentration: 0~1000ng/ml

    Primary antibody concentration range: 1000ng/ml

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)

    Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labelling JAK1 with unpurified ab138005 antibody at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
    OI-RD Scanning - Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138005).

  • Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)
    Anti-JAK1 (phospho Y1022 + Y1023) antibody [EPR1899(2)] - BSA and Azide free (ab203784)

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