Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1593Y] to Integrin linked ILK
Suitable for: WB, IP, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human, African green monkey

Product name

Anti-Integrin linked ILK antibody [EP1593Y]
See all Integrin linked ILK primary antibodies
Description

Rabbit monoclonal [EP1593Y] to Integrin linked ILK
Host species

Rabbit
Specificity

This antibody reacts with Integrin linked ILK. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Rat Human African green monkey

See all applications and species data

Immunogen

Synthetic peptide within Human Integrin linked ILK aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q13418
Positive control
- Human heart muscle tissue and COS cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1593Y
Isotype

IgG
Research areas

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Integrin linked ILK knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab52480 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

Unpurified ab52480 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. Unpurified ab52480 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling Integrin linked ILK with Purified ab52480 at 1/100 dilution (7.13 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0).
Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates with 5% NFDM/TBST
Lane 3 : Mouse heart lysates with 5% NFDM/TBST
Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 51 kDa
Observed band size: 51 kDa
Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

ab52480 (purified) at 1:40 dilution (2µg) immunoprecipitating Integrin linked ILK in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab52480 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52480 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Overlay histogram showing HEK293 cells stained with unpurified ab52480 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab52480, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/1000 dilution (Purified) + COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg with 5% NFDM/TBST

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 51 kDa
Observed band size: 51 kDa
Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution (unpurified) + COS-1 (African green monkey kidney fibroblast-like) cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa
Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) (unpurified) + PC12

Predicted band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Immunohistochemical analysis of paraffin-embedded human heart muscle using unpurified ab52480 at a 1/50 dilution.
Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution (unpurified)

Lane 1 : Rat Brain
Lane 2 : Rat Heart
Lane 3 : Rat Kidney
Lane 4 : Rat Spleen

Predicted band size: 51 kDa

Exposure time: 3 minutes
Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

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