Anti-Integrin linked ILK antibody (ab236455)
Key features and details
- Rabbit polyclonal to Integrin linked ILK
- Suitable for: ICC, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-Integrin linked ILK antibody
See all Integrin linked ILK primary antibodies -
Description
Rabbit polyclonal to Integrin linked ILK -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish -
Immunogen
Recombinant fragment corresponding to Human Integrin linked ILK aa 1-228.
Database link: Q13418 -
Positive control
- WB: HeLa, NIH/3T3 and HL-60 whole cell lysates; Mouse liver and lung lysates; Rat liver and lung lysates; Zebrafish lysate. IHC-P: Human kidney and skeletal muscle tissue; paraffin-embedded HEK-293T cells. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Integrin linked ILK antibody (ab236455)All lanes : Anti-Integrin linked ILK antibody (ab236455) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse lung lysate
Lane 6 : Rat liver lysate
Lane 7 : Rat lung lysate
Lane 8 : Zebrafish lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 51 kDa
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Immunocytochemistry - Anti-Integrin linked ILK antibody (ab236455)Paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (ab236455)Paraffin-embedded human kidney tissue stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (ab236455)Paraffin-embedded human skeletal muscle tissue stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody (ab236455)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Integrin linked ILK (green) using ab236455 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
