Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1593Y] to Integrin linked ILK - Low endotoxin, Azide free
  • Suitable for: IP, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free
    See all Integrin linked ILK primary antibodies

  • Description

    Rabbit monoclonal [EP1593Y] to Integrin linked ILK - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Monkey, African green monkey

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human heart muscle tissue and COS cell lysate

  • General notes

    ab221922 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1593Y

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)
    Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)

    ab52480 (purified) at 1:40 dilution (2µg) immunoprecipitating Integrin linked ILK in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab52480 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52480 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52480).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling Integrin linked ILK with Purified ab52480 at 1:100 dilution (7.13 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52480).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)

    Immunohistochemical analysis of paraffin-embedded human heart muscle using unpurified ab52480 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52480).

  • Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)
    Anti-Integrin linked ILK antibody [EP1593Y] - Low endotoxin, Azide free (ab221922)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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