Anti-Integrin linked ILK antibody (ab227154)
Key features and details
- Rabbit polyclonal to Integrin linked ILK
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin linked ILK antibody
See all Integrin linked ILK primary antibodies -
Description
Rabbit polyclonal to Integrin linked ILK -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Guinea pig, Cow, Rhesus monkey -
Immunogen
Recombinant fragment within Human Integrin linked ILK (internal sequence). The exact sequence is proprietary.
Database link: Q13418 -
Positive control
- WB: WT HeLa cell extracts. PC-12, Neuro-2a, C8D80, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. A431 whole cell lysate. IHC-P: Mouse kidney tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Integrin linked ILK Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
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All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : Integrin linked ILK knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
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Paraffin embedded mouse kidney tissue stained for Integrin linked ILK with ab227154 (1/500 dilution) in immunohistochemical analysis.
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All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extracts
Lane 2 : C3D80 whole cell extracts
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extracts
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extracts
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
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Integrin linked ILK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 4 µg of ab227154. Western blot was performed from the immunoprecipitate using ab227154 at1/500 dilution.
Lane 1: 30 μg HeLa whole cell extract
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: ab227154 IP in HeLa whole cell extracts.
10% SDS-PAGE.
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Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
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Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa7.5% SDS PAGE
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All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C3D80 whole cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa10 % SDS-PAGE