Anti-CD45 antibody [HI30] (ab123522)
![Anti-CD45 antibody [HI30] (ab123522)](https://www.abcam.com/ps/products/123/ab123522/Images/ab123522-199436-anti-cd45-antibody-hi30-flow-cytometry.jpg "Anti-CD45 antibody [HI30] (ab123522)")
Key features and details
- Mouse monoclonal [HI30] to CD45
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD45 antibody [HI30]
See all CD45 primary antibodies -
Description
Mouse monoclonal [HI30] to CD45 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Immunogen information is proprietary
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab123522 was determined to be >90% pure by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
HI30 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD45 antibody [HI30] (ab123522)
Human peripheral blood lymphocytes stained with ab123522 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab123522, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
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![Flow Cytometry - Anti-CD45 antibody [HI30] (ab123522)](https://www.abcam.com/ps/products/123/ab123522/Images/ab123522-455401-AntiCD45-antibody-Flow-cytomerty.jpg)
Flow Cytometry - Anti-CD45 antibody [HI30] (ab123522)Flow cytometry analysis (surface staining) of human peripheral blood cells with ab123522
