Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 09, 2021

Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6741(B)] to IGF2BP2/IMP-2
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)]
See all IGF2BP2/IMP-2 primary antibodies
Description

Rabbit monoclonal [EPR6741(B)] to IGF2BP2/IMP-2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human IGF2BP2/IMP-2.
Positive control
- 293T, Caco2, HepG2, HeLa and SH SY5Y cell lysates, rat brain and mouse heart lysate; Human colon and human lung carcinoma tissue.
General notes
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
Previously labelled as IGF2BP2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 4.00 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6741(B)
Isotype

IgG
Research areas

Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/5000 dilution

Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 66 kDa
Observed band size: 60,62 kDa
why is the actual band size different from the predicted?

Exposure time: 60 seconds

Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBST

The double bands are consistent with what has been described in PMID 23177649.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling IGF2BP2/IMP-2 with purified ab124930 at 1/200 dilution (4.8 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling IGF2BP2/IMP-2 with purified ab124930 at 1/250 dilution (3.8μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor^® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/10000 dilution (purified) + Mouse heart lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/2000 dilution (purified)

Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 66 kDa
Observed band size: 66 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

ab124930 (unpurified), at 1/50 dilution, staining IGF2BP2/IMP-2 in formalin-fixed, paraffin-embedded Human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

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