Anti-IGF2BP2/IMP-2 antibody (ab67434)
Key features and details
- Mouse polyclonal to IGF2BP2/IMP-2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IGF2BP2/IMP-2 antibody
See all IGF2BP2/IMP-2 primary antibodies -
Description
Mouse polyclonal to IGF2BP2/IMP-2 -
Host species
Mouse -
Specificity
This antibody is reactive against mammalian transfected lysate. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length protein corresponding to Human IGF2BP2/IMP-2.
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Positive control
- IGF2BP2/IMP-2 transfected 293T cell lysate and human placenta tissue lysate
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General notes
Previously labelled as IGF2BP2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IGF2BP2/IMP-2 antibody (ab67434)Anti-IGF2BP2/IMP-2 antibody (ab67434) at 1/500 dilution + human placenta tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
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Western blot - Anti-IGF2BP2/IMP-2 antibody (ab67434)All lanes : Anti-IGF2BP2/IMP-2 antibody (ab67434) at 1/500 dilution
Lane 1 : IGF2BP2/IMP-2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 62 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP2/IMP-2 antibody (ab67434)ICC/IF image of ab67434 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67434, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
