Anti-Nanos3 antibody (ab70001)
Key features and details
- Rabbit polyclonal to Nanos3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nanos3 antibody -
Description
Rabbit polyclonal to Nanos3 -
Host species
Rabbit -
Specificity
ab70001 does not cross-react with either Nanos1 or Nanos2. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic 16 amino acid peptide near the carboxy terminus of the human Nanos3 (GenBank accession no. P60323).
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Positive control
- Human brain tissue lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ab70001 at 2.5µg/ml staining Nanos3 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
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Anti-Nanos3 antibody (ab70001) at 2 µg/ml + Human brain tissue lysate at 15 µg
Predicted band size: 19 kDa
Observed band size: 19 kDa
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ICC/IF image of ab70001 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70001, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunofluorescence of Nanos3 in Human Brain cells using ab70001 at 20 ug/ml.