Anti-PERK antibody (ab79483)
Key features and details
- Rabbit polyclonal to PERK
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PERK antibody
See all PERK primary antibodies -
Description
Rabbit polyclonal to PERK -
Host species
Rabbit -
Specificity
ab79483 detects endogenous levels of total PERK protein -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic non-phosphopeptide derived from human PERK around the phosphorylation site of threonine 981 (RHTPGQ)
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Positive control
- IHC: human colon carcinoma tissue WB: extracts from MCF-7 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PERK antibody (ab79483)All lanes : Anti-PERK antibody (ab79483) at 1/500 dilution
Lane 1 : extracts from MCF-7 cells
Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 125 kDa
Observed band size: 125 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PERK antibody (ab79483) This image is courtesy of an anonymous abreview.IHC-P image of PERK staining on human cortical sections using ab79483 (1:500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab79483 was diluted 1:500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1:500)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PERK antibody (ab79483)Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, stained with ab79483 at 1/50-1/100 dilution, in the absence and presence of the immunising peptide
