Anti-PSRC2 antibody [EPR20913-32] (ab236629)
![Anti-PSRC2 antibody [EPR20913-32] (ab236629)](https://www.abcam.com/ps/products/236/ab236629/Images/ab236629-451626-anti-psrc2-antibody-epr2091332-western-blot-lysate.jpg "Anti-PSRC2 antibody [EPR20913-32] (ab236629)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20913-32] to PSRC2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PSRC2 antibody [EPR20913-32]
See all PSRC2 primary antibodies -
Description
Rabbit monoclonal [EPR20913-32] to PSRC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lymph node(WT) and testis tissue lysates; Rat thymus tissue lysate; HeLa; 293T whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20913-32 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PSRC2 antibody [EPR20913-32] (ab236629)All lanes : Anti-PSRC2 antibody [EPR20913-32] (ab236629) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat thymus tissue lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 226 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds
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![Western blot - Anti-PSRC2 antibody [EPR20913-32] (ab236629)](https://www.abcam.com/ps/products/236/ab236629/Images/ab236629-451625-anti-psrc2-antibody-epr2091332-western-blot-tissue.jpg)
Western blot - Anti-PSRC2 antibody [EPR20913-32] (ab236629)All lanes : Anti-PSRC2 antibody [EPR20913-32] (ab236629) at 1/1000 dilution
Lane 1 : Mouse lymph node(WT) tissue lysate
Lane 2 : PSRC2 knockout mouse lymph node tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 226 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were kindly provided by collaborator.
ab236629 Anti-PSRC2 antibody [EPR20913-32] was shown to react with PSRC2 in mouse lymph node tissue lysate in Western blot. Loss of signal was observed when PSRC2 knockout sample was used. Wild-type and PSRC2 knockout samples were subjected to SDS-PAGE. ab236629 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. he blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 15 seconds